| 2021-05-18 07:00:30Tuesday, May 18, 2021 | Plymouth Gladiators v Glasgow Tigers | 07:00:30Postponed | P - P | Championship | Plymouth Coliseum |
| 2021-05-21 15:12:26Friday, May 21, 2021 | Scunthorpe Scorpions v Plymouth Gladiators | 15:12:26Postponed | P - P | Championship | Eddie Wright Raceway |
| 2021-05-23 15:00:26Sunday, May 23, 2021 | Scunthorpe Scorpions v Plymouth Gladiators | 15:00:263:00 pm | 56 - 34 | Championship | Eddie Wright Raceway |
| 2021-05-25 00:05:30Tuesday, May 25, 2021 | Plymouth Gladiators v Poole Pirates | 00:05:30Postponed | P - P | Championship KO Cup, 1st Round, 1st Leg | Plymouth Coliseum |
| 2021-06-01 19:00:30Tuesday, June 1, 2021 | Plymouth Gladiators v Kent Kings | 19:00:307:00 pm | 45 - 45 | Championship | Plymouth Coliseum |
| 2021-06-08 19:00:30Tuesday, June 8, 2021 | Plymouth Gladiators v Berwick Bandits | 19:00:307:00 pm | 51 - 39 | Championship | Plymouth Coliseum |
| 2021-06-15 18:30:59Tuesday, June 15, 2021 | Kent Kings v Plymouth Gladiators | 18:30:596:30 pm | 49 - 41 | Championship | Central Park Stadium |
| 2021-06-22 19:00:32Tuesday, June 22, 2021 | Plymouth Gladiators v Poole Pirates | 19:00:327:00 pm | 41 - 49 | Championship KO Cup, 1st Round, 1st Leg | |
| 2021-06-27 00:05:55Sunday, June 27, 2021 | Newcastle Diamonds v Plymouth Gladiators | 00:05:55Postponed | P - P | Championship | Newcastle Stadium |
| 2021-06-29 00:05:30Tuesday, June 29, 2021 | Plymouth Gladiators v Newcastle Diamonds | 00:05:30Postponed | P - P | Championship | Plymouth Coliseum |
| 2021-07-02 19:30:24Friday, July 2, 2021 | Glasgow Tigers v Plymouth Gladiators | 19:30:24Postponed | P - P | Championship | Peugeot Ashfield Stadium |
| 2021-07-13 19:00:30Tuesday, July 13, 2021 | Plymouth Gladiators v Poole Pirates | 19:00:307:00 pm | 45 - 45 | Championship | Plymouth Coliseum |
| 2021-07-20 19:00:12Tuesday, July 20, 2021 | Plymouth Gladiators v Glasgow Tigers | 19:00:127:00 pm | 44 - 46 | Championship | Plymouth Coliseum |
| 2021-07-21 19:30:19Wednesday, July 21, 2021 | Poole Pirates v Plymouth Gladiators | 19:30:197:30 pm | 61 - 29 | Championship KO Cup, 1st Round, 2nd Leg | Poole Stadium |
| 2021-07-27 19:00:30Tuesday, July 27, 2021 | Plymouth Gladiators v Leicester Lions | 19:00:307:00 pm | 43 - 46 | Championship | Plymouth Coliseum |
| 2021-07-28 11:00:30Wednesday, July 28, 2021 | Birmingham Brummies v Plymouth Gladiators | 11:00:30Postponed | P - P | Championship | Perry Barr Stadium |
| 2021-07-31 19:00:57Saturday, July 31, 2021 | Eastbourne Eagles v Plymouth Gladiators | 19:00:577:00 pm | 54 - 36 | Championship | Arlington Stadium |
| 2021-08-03 19:00:30Tuesday, August 3, 2021 | Plymouth Gladiators v Edinburgh Monarchs | 19:00:307:00 pm | 50 - 40 | Championship | Plymouth Coliseum |
| 2021-08-08 18:00:58Sunday, August 8, 2021 | Newcastle Diamonds v Plymouth Gladiators | 18:00:586:00 pm | 45 - 33 | Championship | Newcastle Stadium |
| 2021-08-10 19:00:30Tuesday, August 10, 2021 | Plymouth Gladiators v Redcar Bears | 19:00:307:00 pm | 46 - 43 | Championship | Plymouth Coliseum |
| 2021-08-13 19:30:59Friday, August 13, 2021 | Edinburgh Monarchs v Plymouth Gladiators | 19:30:597:30 pm | 47 - 42 | Championship | Armadale Stadium |
| 2021-08-14 19:00:55Saturday, August 14, 2021 | Berwick Bandits v Plymouth Gladiators | 19:00:557:00 pm | 57 - 33 | Championship | Shielfield Park |
| 2021-08-15 14:00:02Sunday, August 15, 2021 | Redcar Bears v Plymouth Gladiators | 14:00:022:00 pm | 55 - 35 | Championship | Media Prima Arena |
| 2021-08-17 19:00:30Tuesday, August 17, 2021 | Plymouth Gladiators v Scunthorpe Scorpions | 19:00:307:00 pm | 47 - 37 | Championship | Plymouth Coliseum |
| 2021-08-24 19:00:08Tuesday, August 24, 2021 | Plymouth Gladiators v Birmingham Brummies | 19:00:087:00 pm | 47 - 42 | Championship | Plymouth Coliseum |
| 2021-08-25 19:30:54Wednesday, August 25, 2021 | Birmingham Brummies v Plymouth Gladiators | 19:30:547:30 pm | 54 - 36 | Championship | Perry Barr Stadium |
| 2021-08-28 19:00:16Saturday, August 28, 2021 | Leicester Lions v Plymouth Gladiators | 19:00:167:00 pm | 50 - 40 | Championship | Paul Chapman & Sons Arena |
| 2021-08-31 19:00:47Tuesday, August 31, 2021 | Plymouth Gladiators v Newcastle Diamonds | 19:00:477:00 pm | 48 - 42 | Championship | Plymouth Coliseum |
| 2021-09-07 19:00:36Tuesday, September 7, 2021 | 90th Anniversary @ Plymouth | 19:00:367:00 pm | 1st - Danny King | 90th Anniversary | Plymouth Coliseum |
| 2021-09-08 19:30:22Wednesday, September 8, 2021 | Poole Pirates v Plymouth Gladiators | 19:30:227:30 pm | 55 - 35 | Championship | Poole Stadium |
| 2021-09-10 19:30:21Friday, September 10, 2021 | Glasgow Tigers v Plymouth Gladiators | 19:30:217:30 pm | 59 - 31 | Championship | Peugeot Ashfield Stadium |
